Development of Sensitive Optical Biosensing Technologies for Biomedical Applications
Speaker: Jingyong Ye (Department of Biomedical Engineering, the University of Texas at San Antonio)
Time: June 22, 2017 16:00
There is a high demand for highly sensitive biosensors for quantifying biomolecule concentrations or binding affinities for a wide range of biomedical and pharmaceutical applications. In this talk I will present two unique biosensors for both in vitro and in vivo applications. First, I will describe a photonic crystal based biosensor for label-free bioassays. As opposed to a conventional optical microcavity sensor that has a closed structure with its cavity layer sandwiched between two highly reflective surfaces, the photonic crystal sensor we developed possesses a novel open optical microcavity. The open configuration allows the sensing layer to be easily functionalized and directly exposed to analyte molecules for label-free bioassays through monitoring the change in resonant conditions of the open microcavity. A wide range of applications can be facilitated by the unique open-microcavity biosensor. In particular, two different applications will be covered in this talk. One is for label-free measurements of cardiac biomarkers- Troponin I (cTnI). The sensor is functionalized with antibodies against cTnI for sensitive detection of cTnI with a concentration as low as 0.1 ng mL-1. The other application is to use the sensor for the study of interactions between different peptides and FvTox1- a toxin produced by a soil borne pathogen that causes sudden death syndrome in soybean plants. Furthermore, I will discuss the development of a novel two-photon optical fiber fluorescence (TPOFF) probe and its applications for in vivo biosensing, such as real-time monitoring nanoparitcle-based drug delivery in a live mouse model.
Bio: 叶竞涌博士现为德克萨斯大学圣安东尼奥分校生物医学工程系的终身教授，叶教授长期从事激光生物技术和生物纳米技术的研究。他领导了多个重大研究项目，包括光子晶体生物分子检测、光纤生物传感和成像系统监测多功能纳米材料在体跟踪治癌药物的靶向释放、超快激光与纳米微粒定位的癌细胞的相互作用、超高频率的光声成像传感器的研发、多光子光纤扫描显微镜、活体双光子流式细胞仪、共聚焦显微镜中的自适应光学畸变校正、单分子荧光成像和光谱。叶教授在国际期刊及会议上发表论文二百三十余篇，并有13项专利。叶教授是美国国立卫生研究院(NIH)、美国国家科学基金会(NSF)、美国食品药品监督管理局(FDA)，以及数家私人基金会的基金项目的评审专家，是近30个科学杂志的审稿人。2002年成为IEEE的高级会员，曾任美国光学学会-安娜堡分会会长,现任美国光学学会的Molecular Probes and Nanobio-Optics技术委员会的副主席。此外，他是Photon Affinity公司的创始人之一，还是另一家生物高科技公司的顾问委员会成员，并为五家公司担任专业顾问。